Nitric Oxide and Depolarization Induce Hydroxyl Radical Generation
نویسندگان
چکیده
منابع مشابه
Depolarization of mitochondria in neurons promotes activation of nitric oxide synthase and generation of nitric oxide.
The diverse signaling events following mitochondrial depolarization in neurons are not clear. We examined for the first time the effects of mitochondrial depolarization on mitochondrial function, intracellular calcium, neuronal nitric oxide synthase (nNOS) activation, and nitric oxide (NO) production in cultured neurons and perivascular nerves. Cultured rat primary cortical neurons were studied...
متن کاملHOCl-dependent singlet oxygen and hydroxyl radical generation modulate and induce apoptosis of malignant cells.
The lack of extracellular superoxide anion production by non-transformed cells prevents H2O2/peroxidase-mediated HOCl synthesis by these cells, as well as apoptosis induction by exogenous HOCl. In contrast, transformed cells generate extracellular superoxide anions and HOCl, and die by apoptosis after HOCl/superoxide-dependent hydroxyl radical generation at their membrane. Tumor cells prevent H...
متن کاملApparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide.
Superoxide dismutase reduces injury in many disease processes, implicating superoxide anion radical (O2-.) as a toxic species in vivo. A critical target of superoxide may be nitric oxide (NO.) produced by endothelium, macrophages, neutrophils, and brain synaptosomes. Superoxide and NO. are known to rapidly react to form the stable peroxynitrite anion (ONOO-). We have shown that peroxynitrite ha...
متن کاملHuman granulocyte generation of hydroxyl radical
Human granulocytes were capable of oxidizing 2-keto-4 thiomethylbutyric acid to ethylene during phagocytosis or membrane perturbation. The reaction required hydrogen peroxide and superoxide and in addition was inhibited by various hydroxyl radical (OH) scavengers. These observations represent direct evidence for the generation of OH by human granulocytes. Further, inhibition of ethylene generat...
متن کاملHydroxyl radical generation by photosystem II.
The photogeneration of hydroxyl radicals (OH(*)) in photosystem II (PSII) membranes was studied using EPR spin-trapping spectroscopy. Two kinetically distinguishable phases in the formation of the spin trap-hydroxyl (POBN-OH) adduct EPR signal were observed: the first phase (t(1/2) = 7.5 min) and the second phase (t(1/2) = 30 min). The generation of OH(*) was found to be suppressed in the absen...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 2002
ISSN: 0021-5198
DOI: 10.1254/jjp.88.1